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The two protein bands from 40 kDa to thirty kDa had been absent just after 24 h incubation at 45 °C. For the rest of the bands, a variety of concentrations of all three proteases retained protein bands comparable to their first molar masses (Determine 6a,b). The presence of bands at very low molecular bodyweight in fish roe ahead of hydrolysis was unexpected. While in the report by Ghelichi et al. [eight], insignificant peaks of a relatively lower molecular weight range (3–fourteen kDa) were being observed in defatted carp roe homogenate, potentially resulting from an autolytic process developing in fish roe and/or the defatting procedure resulting in the development of reduced-molecular-fat peptides.
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